Effect of Azospirillum brasilense and Bacillus subtilis Inoculation on Durum Wheat Growth Response under Four Inoculation Methods

Effect of Azospirillum brasilense and Bacillus subtilis Inoculation on Durum Wheat Growth Response under Four Inoculation Methods

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The growth response of durum wheat (Triticum durum) to inoculation by two rhisospheric rhizobacteria: Azospirillum brasilense and Bacillus subtilis was evaluated using four ways of inoculation read more to determine the best method that gives better results.The two rhizobacteria were inoculated on LB liquid medium.Durum wheat was inoculated twice.For the first inoculation, part of the germinated seeds was directly sown on the ground and inoculated by bacterial pellet (PP) or by medium containing bacterial culture (MM).As for the other part of the germinated seeds, they were first immerged for 45 min in a medium containing the bacterial culture, after that, they were sown on the ground.

For the second inoculation, it was carried out 10 days after sowing, by the same method, except for the seeds being immerged in the culture medium, which were inoculated this time by bacterial pellet (IP) or by medium containing bacterial culture (IM).After 3 weeks of growth, different plant parameters such as the fresh and dry weight of leaves and roots, the number of leaves, the length of leaves and roots and the chlorophyll levels were compared between inoculated and non-inoculated plants and according to the different inoculation methods.The results demonstrated that the inoculation aptamil allerpro of durum wheat with these two strains stimulated the growth of the plant, some parameters gave similar effects between the two bacteria and other parameters gave different effects.Similarly, the type of inoculation influenced the response of the plant to the bacterium; some types gave better results compared to others.